Prevalence and molecular identification of Sarcocystis spp.infecting water buffaloes (Bubalus bubalus) in Sharkia Province, Egypt.

Sarcocystosis is regarded as one of the most important protozoal infections buffaloes. This study was conducted to determine prevalence Sarcocystis spp. in water buffaloes (Bubalus bubalis) slaughterhouses Sharkia province, Egypt. In addition, effect age and sex on infection rates reported. Therefore, isolates were identified morphologically using macroscopic, microscopic histopathological examinations, while molecular identification carried out amplification 18S ribosomal subunit RNA (18S rRNA) by PCR DNA sequencing. The current findings showed that 41.50%. Further, both morphological sequences analysis indicated obtained from macroscopic S. fusiformis. first genetic fusiformis slaughtered province which will provide useful information for monitoring controlling infections.